Notes: (A and B) Si-NC or si-XIST was transfected into AMC-HN-8 and M4E cells, and the interference effects were detected by qRT-PCR at 48 h post transfection. (C and D) Cell proliferation was examined by CCK-8 assay and (E and F) flow cytometry was used to detect cell apoptosis. (G and H) Transwell assay was conducted to measure migration and invasion of transfected cells; *P <0.05.