(A) SKA3 silencing (KD, shSKA3) in the indicated cell lines. (B–D) Effects of SKA3 silencing on cell proliferation (B). A one way ANOVA was used for data comparisons. (C and D) Migration and invasion assays of H226 and SK-MES-1 cells, respectively. Data were compared via a Student’s t test. (E) H & E staining of mouse lung tissues from H226-shNT and H226-shSKA3 groups (40×, metastatic nodules are indicated by arrows). (F) Numbers of metastatic foci observed in each group (n = 5). Data were analyzed through a Student’s t-test. *P<0.05; **P<0.01.