HUVECs cultured in hypoxia for 24 h had significantly up-regulated JMJD6, with no change in U2AF65 expression (A). Similarly, BeWo cells exposed to hypoxia also had unchanged U2AF65 but significantly elevated JMJD6 (B). Chronic hypoxia of the RUPP placenta, however, had significantly increased U2AF65 (C) and JMJD6 (D) compared with sham animals. Two-way ANOVA was performed for both cell groups. Student’s t-test was performed on the placental protein data. N = 6 in all groups. *P < 0.05; #P < 0.01 ##P < 0.001 vs. control/ sham.