Effect of U2AF65 and JMJD6 expression on heparanase
HUVEC knockdown samples showed that there was no effect on heparanase mRNA expression (N = 4) (A) or media protein (N = 3) (B). HUVEC JMJD6 overexpression had significant increase in heparanase mRNA (N = 4) (C), but only HUVEC overexpression of U2AF65 had significant increase in media heparanase protein (N = 3) (D). BeWo knockdown of JMJD6 showed significantly decreased heparanase message (N = 6) (E), but none of the BeWo knockdown groups showed any change in heparanase media protein (N = 3) (F). BeWo overexpression of U2AF65 and JMJD6 had no effect on heparanase mRNA (N = 6) (G) or media protein (N = 3) (H). One-way ANOVE was performed for all groups. *P < 0.05 vs. control.