HUVEC knockdown (A) and overexpression (B) samples were analyzed for impact on sFlt-1 mRNA, with JMJD6 overexpression causing significantly increased sFlt-1 version 3 expression (N = 4). Media from HUVEC samples were analyzed for sFlt-1 protein. Both knockdown (C) and overexpression (D) media showed trends of sFlt-1 protein in the same direction as U2AF65 and JMJD6 expression (N = 4). BeWo knockdown (E) or overexpression (F) had no effect on sFlt-1 mRNA (N = 6). Similarly, media collected from U2AF65 or JMJD6 knockdown (G) or overexpression (H) had no difference in media sFlt-1 concentration (N = 6). Two-way ANOVA was performed on the rtPCR samples, and one-way ANOVA was performed on media sFlt-1 samples *P < 0.05 vs. control. ND = Not detectable.