rtPCR was performed to ensure adequate knockdown (A) and overexpression (B) was achieved in BeWo placental trophoblasts (N = 6). Additionally, Western blots were performed to see if the message translated to the protein expression. U2AF65 and JMJD6 knockdown Western blots and their analyses are shown in (C), while overexpression can be observed in (D) (N = 3). *P < 0.05; #P < 0.01