Figure 2
(A) Overlays of an expanded region of the 1H,15N-HSQC spectrum of Eaf3 CD titrated with H3K36me3 peptide. (B) Titration curves for the side chain imino groups from Trp84 and Trp88 of Eaf3 CD. The added histone peptides and amino acids are shown on the right. Kme3: tri-methyl-lysine, Rame2: asymmetrically di-methylated arginine.
NMR titration of Eaf3 CD with K4- or K36-methylated histone H3 peptide

(A) Overlays of an expanded region of the 1H,15N-HSQC spectrum of Eaf3 CD titrated with H3K36me3 peptide. (B) Titration curves for the side chain imino groups from Trp84 and Trp88 of Eaf3 CD. The added histone peptides and amino acids are shown on the right. Kme3: tri-methyl-lysine, Rame2: asymmetrically di-methylated arginine.

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