Cells were treated with pyrotinib for 24 h or/and 6-Gy irradiation. After 24 h, cells were harvested and subjected to flow cytometry. (A) Flow cytometry results of cell cycle analysis of TE-1 cells. (B) Flow cytometry results of cell cycle analysis of KYSE30 cells. (C) Percent of cells in different cycle phase. *P<0.05; **P<0.01; ***P<0.001 compared with control group. #P<0.05; ##P <0.01 compared with radiation group.