HK-2 cells were randomly divided into four groups: control, ABL, IOP, and ABL + IOP groups. Cells in the ABL + IOP group were pretreated with ABL (100 μM) for 2 h and then incubated with IOP (80 mg I/ml) for 1 h. Cells in ABL or IOP groups were given ABL for 2 h or IOP for 1 h, respectively. The control group was administered with the same amount of vehicle. (A,B) The percentage of caspase-1 (+) PI (+) cells of four groups was determined by flow cytometry. (C) Cell viability was examined by CCK-8 kits. (D,E) IL-1β and IL-18 levels were detected by ELISA kits. (F) Shows the LDH release activity of the four groups. (G–Q) Protein or total RNA was extracted for Western blot or RT-PCR. The protein levels of NLRP3, ASC, caspase-1, GSDMD, and IL-1β were normalized to GAPDH. The mRNA levels of NLRP3, ASC, caspase-1, GSDMD, and IL-1β were normalized to GAPDH. Compared with the control group, ^aP<0.01 and ^bP<0.01 in the ABL+IOP group vs IOP group.