IOP blocked the expression of proteins related to pyroptosis in dosage- and time-dependent ways
(A) HK-2 cells were incubated with IOP at a concentration of 0–160 mg I/ml for 2 h, respectively. Proteins were extracted for Western blot. The relative expression of NLRP3 (B), ASC (C), cleaved caspase-1 (D), mature GSDMD (E), and IL-1β (F) was normalized to GAPDH. (G) HK-2 cells were incubated with IOP (80 mg I/ml) for 0–120 min, respectively. Proteins were isolated for Western blot. The relative expression of NLRP3 (H), ASC (I), cleaved caspase-1 (J), mature GSDMD (K), and IL-1β (L) was calculated by the normalization to GAPDH. *P<0.05, **P<0.01.