Immunofluorescence staining of the ET-1-induced cardiomyocytes (hiPSC-CM)
(A) After induction of hiPSCs with 10 nM ET-1 (middle panels) for 24 h, expression of BNP (red) and α-actinin (green) were enhanced, and the cell shape was increased compared with the non-treated cells (up panels) and Bosentan (ET-1 antagonist) treated cells (lower panels); scale bar: 40 μm. (B) The levels of proBNP and α-actinin were significantly increased in the ET-1 group compared with the control group. However, Bosentan treatment reduced the expression of proBNP and α-actinin; however, both were still expressed at lower levels compared with the control group. *: P < 0.05 compared with control group.