(A) Expression of CTLA-4 was subjected to regulation of pcDNA3.1-MALAT1 and si-MALAT1-3. *: P<0.05 when compared with NC. (B) The expression of MALAT1 remained stable under the transfection of pcDNA3.1-CTLA-4 or si-CTLA-4-1. *: P<0.05 when compared with NC. (C) The mRNA levels of IFN-γ, IL-2 and T-bet within CD4+ T cells were compared among pcDNA3.1-CTLA-4+si-MALAT1-3, si-MALAT1-3 and NC groups. *: P<0.05 when compared with NC group. (D) The mRNA levels of IL-4, IL-10 and GATA3 were drawn from CD4+ T cells transfected by pcDNA3.1-CTLA-4+si-MALAT1-3, si-MALAT1-3 and NC. *: P<0.05 when compared with NC. (E) The protein levels of IFN-γ, IL-2 and T-bet were obtained from CD4+ T cells transfected by pcDNA3.1-CTLA-4+si-MALAT1-3, si-MALAT1-3 and NC. *: P<0.05 when compared with NC. (F) The protein levels of IL-4, IL-10 and GATA3 were determined among pcDNA3.1-CTLA-4+si-MALAT1-3, si-MALAT1-3 and NC groups. *: P<0.05 when compared with NC.