The script processes phases II and IV automatically. Phase I is done manually by the user, organizing the input file according to what is required: accession numbers, protein and gene names. Phase II (retrieval) is automatic, and the data is retrieved from UniProtKB. Phase III relies on the PROMALS3D server, in which the input data need to be manually organized and submitted. Phase IV is based on the PROMALS3D alignment results, where the ER-retrieval signal consensus is searched through all proteins in the list automatically. Examples of possible output data shown.