The BV2 cells were treated with LPS (1 µg/ml), and transfected with Ctrl-siRNA, si-PTX3 respectively. and the levels of pro-inflammatory factors were detected by ELISA and Western blot. (A) The protein expression of PTX3 in the control, LPS, Ctrl-siRNA and si-PTX3 group. (B-D) The level of IL-6, NO and TNF-α in the control, LPS, Ctrl-siRNA and si-PTX3 group. (E,F) The protein expression of COX-2 and iNOS in the control, LPS, Ctrl-siRNA and si-PTX3 group. ‘*’ means compared with the control group at P<0.05, and ‘#’ means compared with the LPS+Ctrl-siRNA group at P<0.05. GAPDH was used as an invariant internal control for calculating protein fold changes.