(A) The mRNA expression of PTX3 in mice treated with LPS (200 ng in 25 μl of sterile saline) at 1, 3, 5 and 7 days. (B) The protein expression of PTX3 in mice treated with LPS (200 ng in 25 μl of sterile saline) at 1, 3, 5 and 7 days. (C) MWT after injection. (D) Thermal withdrawal latencies after injection; (E) Frequency responses to cold stimulation after injection. ‘*’ means compared with the control group at P<0.05, and ‘#’ means compared with the LPS+Normal group at P<0.05. GAPDH was used as an invariant internal control for calculating protein fold changes.