(A) Venn diagram shows the overlap between 30 and 20 miRNAs that were differentially altered (P < 0.05) between women with history of PE versus NT pregnancy in cohorts 1 (n = 17–18/group) and 2 (n = 20/group), respectively. Right panel shows the identity of the 4 overlapping miRNAs with their direction and magnitude of change (bottom axis scale) and log10 transformed P-values (diamonds, top axis scale) separated by exposure cohort (ACS cohort 1, C1; non-ACS cohort 2, C2). Dashed vertical line denotes P = 0.05 threshold. Plasma miRNA levels are shown as mean counts per million mapped reads (CPM). Identities of all miRNAs and FDR-adjusted p-values are shown in Supplementary Table S4. (B) Venn diagram shows overlap among differentially altered miRNAs associated with prior PE in ACS subjects (C1: 30 miRs P < 0.05), prior PE in non-ACS subjects (C2; 20 miRs P < 0.05), and current ACS exposure (C3; 259 miRs FDR < 0.05, n = 35–40/group). Right panels show the identity, fold-change, P-values and CPM plasma level for miRNAs that intersect multiple exposure comparisons. Identities of all miRNAs and FDR-adjusted P-values are shown in Supplementary Table S5. (C) Venn diagram shows overlap among differentially altered miRNA candidates associated with prior PE in ACS (C1) or non-ACS (C2) subjects, and 104 miRNAs (C4) previously reported to be altered in circulation during PE [10]. Right panels show details on overlapping miRNAs. P-values and CPM levels for previously published data are not shown. Identities of all miRNAs and FDR-adjusted P-values are shown in Supplementary Table S7.