Figure 5
(A) Representative blots and quantitative results for LC3 and β-MHC protein expression in H9c2 cells in each group (n = 4 per group). (B) ANP and β-MHC mRNA expression in H9c2 cells in each group. (C) Representative imaging of immunostaining H9c2 cells for α-actinin (green) in each group. (D) Quantification of cell surface area (n = 50+ cells per group); *P < 0.05, ***P < 0.0005.
Autophagy inhibition by CQ counteracts the protective effect of CRA on cardiomyocyte hypertrophy

(A) Representative blots and quantitative results for LC3 and β-MHC protein expression in H9c2 cells in each group (n = 4 per group). (B) ANP and β-MHC mRNA expression in H9c2 cells in each group. (C) Representative imaging of immunostaining H9c2 cells for α-actinin (green) in each group. (D) Quantification of cell surface area (n = 50+ cells per group); *P < 0.05, ***P < 0.0005.

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