MTT assay was used to test the viability of cells treated with etomoxir (A) and ST1326 (B) at different concentrations. (C) Increased lipid accumulation and (D) elevated FFA level was caused by etomoxir in BCa UM-UC-3 cells. The (E) ATP and (F) NADPH levels were decreased, and (G) NADP+/NADPH ratio was increased in UM-UC-3 cells. (H) The influence of etomoxir on cell survival was detected by clonogenic survival assay, (I) and alterations of fatty acid content were detected by GC-FID. (J) Statistical analysis of (H). (K) Clonogenic survival assay was performed to detect the influence of ST1326 on BCa cells, (L) confirmed by statistical analysis. Cell types and drug concentrations are indicated. Representative images were from three iterations. *P<0.05, **P<0.01, ***P<0.001.