Effect of losartan on the levels of inflammation and oxidase stress in PVN of prenatal cold exposed offspring at 12 weeks of age
(A–C) The levels of the pro-inflammatory cytokines IL-1β (A), IL-6 (B) and TNF-α (C) were measured by ELISA kits (*P<0.05 vs others, n=8). (D,E) The ROS level was measured by DHE staining and NADPH oxidase activity kit (*P<0.05 vs others, n=5). (F,G) MRS values of cold-exposed offspring with or without losartan. The ratios of NAA/Cr (F) and Cho/Cr (G) of the PVN region were acquired by the MRS to detect the central SNS disorder (*P<0.05 vs prenatal cold treated group, n=6).