Interaction of ANXA2 with p11 and exosomal secretion was involved in surface expression of ANXA2

(A) A549 cells and 16HBE cells were treated with or without 40 μg/ml bleomycin for 48 h. The secreted and exosomal ANXA2 in recovered culture supernatants, as well as the expression of p11 in total cell lysates were detected by Western blot analysis (top). Total lysates were immunoprecipitated with ANXA2 antibody, and immunoblotted with ANXA2 antibody or p11 antibody (bottom). (B) Cells were pretreated with inhibitors MβCD for 1 h, and then treated with or without 40 μg/ml bleomycin for 48 h. The surface ANXA2 from the EGTA eluates was detected by Western blot analysis. (C) Immunofluorescent detection of p11 on the surface of A549 cells and 16HBE cells treated with or without 40 μg/ml bleomycin for 48 h. Microscope magnification: Σ 400×.