(A) β-catenin was over-expressed in shENO1-1 T24 cells and the cells (shCtrl, shENO1-1, shENO1-1+CTNNB) were subjected to Western blot analysis of β-catenin. (B) shCtrl, shENO1-1 and shENO1-1 with over-expressed β-catenin T24 cells were subjected to CCK analysis of proliferation. ***P<0.001. (C) The cells described in B were subjected to colony formation analysis. Left, representative images. Right, quantification results. ***P<0.001. (D and E) qRT-PCR results of cyclin D1 and E1 (D), caspase 3 and 9 (E) in the cells described in B. *P<0.05, **P<0.01. (F) β-catenin was silenced in ENO1 over-expressed T24 cells and the cells (Ctrl, ENO1, ENO1+shCTNNB) were subjected to Western blot analysis of β-catenin. (G) Ctrl, ENO1 and ENO1+shCTNNB T24 cells were subjected to CCK analysis of proliferation. **P<0.01. (H) The cells described in B were subjected to colony formation analysis. Left, representative images. Right, quantification results. *P<0.05, **P<0.01, ***P<0.001.