ENO1 expression is increased in BC tissues and cells
(A) qRT-PCR analysis of ENO1 in BC and normal tissues. ENO1 expression in BC tissues was normalized to ENO1 expression in normal tissues. n = 19 in normal, n = 19 in cancer. ***P<0.001. (B) Western blot analysis of ENO1 in bladder cancer and adjacent normal tissues. (C) mRNA level of ENO1 in bladder cancer and normal tissues that were analyzed from TCGA database. TPM, transcripts per million. P<0.001. (D) qRT-PCR and Western blot analysis of ENO1 in bladder epithelial cells SV-HUC-1 and in BC cells T24, 5637, J82, SW780 and RT4. ENO1 expression in T24, 5637, J82, SW780 and RT4 was normalized to ENO1 expression in SV-HUC-1 cells. *P<0.05, **P<0.01. (E) The overall survival of bladder cancer patients who were divided into ENO1 high- and low-expression groups that were analyzed from TCGA database. n = 204 in low-expression group, n = 205 in high-expression group. P=0.016.