(A) Negative Staining electron micrograph of L. monocytogenes EVs after Optiprep gradient separation (as prepared in [30]). (B) Transmission electron micrograph of EVs from C. neoformans (as prepared in [58]). (C) Scanning electron micrograph of EVs from C. neoformans (as prepared in [58]). Scale bars 100 nm. Images courtesy of Carolina Coelho and Raghav Vij (A), and Julie M. Wolf (B and C).