Portland Press

Figure 5

SH-SY5Y cells were treated with 200 µM of ZnSO4 for 1 h in the absence or presence of emodin (20 µM), or/and A769662. A769662 is an activator of the AMPK signaling pathway. Western blot analysis results to document phosphorylation of ACC (A) and AMPK (B) after zinc treatment. (C) The cell viability rate was determined using the water-soluble tetrazolium salt (WST-8). (D) LDH release was measured in the same experimental conditions and expressed as a percent of total LDH activity after complete cell lysis. (E) The percentage of apoptotic cells was determined using an annexin V-FITC/PI apoptosis detection kit. (F) The percentage of apoptotic cells was determined using an ApoBrdU DNA fragmentation assay kit. The columns were presented as the mean ± SEM (n≥3). *P<0.05 vs. untreated SH-SY5Y cells or SH-SY5Y cells treated with emodin alone. #P<0.05 vs. SH-SY5Y cells treated with zinc alone. &P<0.05 vs. SH-SY5Y cells treated with zinc and emodin.

Zinc-induced AMPK activation was inhibited by emodin

SH-SY5Y cells were treated with 200 µM of ZnSO₄ for 1 h in the absence or presence of emodin (20 µM), or/and A769662. A769662 is an activator of the AMPK signaling pathway. Western blot analysis results to document phosphorylation of ACC (A) and AMPK (B) after zinc treatment. (C) The cell viability rate was determined using the water-soluble tetrazolium salt (WST-8). (D) LDH release was measured in the same experimental conditions and expressed as a percent of total LDH activity after complete cell lysis. (E) The percentage of apoptotic cells was determined using an annexin V-FITC/PI apoptosis detection kit. (F) The percentage of apoptotic cells was determined using an ApoBrdU DNA fragmentation assay kit. The columns were presented as the mean ± SEM (n≥3). *P<0.05 vs. untreated SH-SY5Y cells or SH-SY5Y cells treated with emodin alone. ^#P<0.05 vs. SH-SY5Y cells treated with zinc alone. ^&P<0.05 vs. SH-SY5Y cells treated with zinc and emodin.

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