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Figure 4

SH-SY5Y cells were treated with 200 µM ZnSO4 for 1 h in the absence or presence of emodin (20 µM). (A) Detection of ROS content using fluorescent probe DCFH-DA. Expression of CHOP (B), GADD34 (C), and ATF4 (D) were analyzed by RT-qPCR. The columns were presented as the mean ± SEM (n≥3). *P<0.05 vs. untreated SH-SY5Y cells or SH-SY5Y cells treated with emodin alone. #P<0.05 vs. SH-SY5Y cells treated with zinc alone.

Emodin against zinc-induced production of reactive oxygen species (ROS) and endoplasmic reticulum-stress

SH-SY5Y cells were treated with 200 µM ZnSO₄ for 1 h in the absence or presence of emodin (20 µM). (A) Detection of ROS content using fluorescent probe DCFH-DA. Expression of CHOP (B), GADD34 (C), and ATF4 (D) were analyzed by RT-qPCR. The columns were presented as the mean ± SEM (n≥3). *P<0.05 vs. untreated SH-SY5Y cells or SH-SY5Y cells treated with emodin alone. ^#P<0.05 vs. SH-SY5Y cells treated with zinc alone.

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