Effects of emodin on Zn2+-induced NAD+ depletion and ATP depletion
SH-SY5Y cells were pretreated with or without emodin (20 µM) for 24 h, and then SH-SY5Y cells were exposed to ZnSO4 (200 µM) for 1 h in serum-free medium. After Zn2+ exposure, intracellular NAD+ (A) and ATP (B) contents were measured. Neuronal ATP content was expressed as a percent of the amount of ATP measured in SH-SY5Y cells exposed to sham treatment. The columns were presented as the mean ± SEM (n≥3). *P<0.05 vs. untreated SH-SY5Y cells or SH-SY5Y cells treated with emodin alone. #P<0.05 vs. SH-SY5Y cells treated with zinc alone.