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Figure 2

SH-SY5Y cells were pretreated with or without emodin (20 µM) for 24 h, and then the cells were exposed to ZnSO4 (200 µM) for 1 h in serum-free medium. Concentration of Zn2+ (A) was measured as described in ‘Materials and methods’ section. The expression of zinc transporter families MT1 (B), MT2 (C), and ZnT-1 (D) was analyzed by RT-qPCR. The columns were presented as the mean ± SEM (n ≥ 3). *P<0.05 vs. untreated SH-SY5Y cells or SH-SY5Y cells treated with emodin alone. #P<0.05 vs. SH-SY5Y cells treated with zinc alone.

Emodin (20 μM) suppressed Zn²⁺ influx into SH-SY5Y cells

SH-SY5Y cells were pretreated with or without emodin (20 µM) for 24 h, and then the cells were exposed to ZnSO₄ (200 µM) for 1 h in serum-free medium. Concentration of Zn²⁺ (A) was measured as described in ‘Materials and methods’ section. The expression of zinc transporter families MT1 (B), MT2 (C), and ZnT-1 (D) was analyzed by RT-qPCR. The columns were presented as the mean ± SEM (n ≥ 3). *P<0.05 vs. untreated SH-SY5Y cells or SH-SY5Y cells treated with emodin alone. ^#P<0.05 vs. SH-SY5Y cells treated with zinc alone.

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