After HGC-27 cells were transfected with siPELI2, siRNA control, PELI2 plasmid or empty plasmid, the viability of HGC cells was determined by CCK-8 (A); the colony formation ability of the cells was evaluated by colony formation assay (B); the migration (C) and invasion (D) of the cells were detected by transwell assay. *P<0.05 versus control.