(A) Schematic representation of mechanical stretch is shown. (B) H-ASMCs pretreated with SKF96365 (0.4 μM) for 6 h then mechanically stretched for 2 or 4 h. F-actin stained by phalloidin; n=3. (C) Apoptosis of H-ASMCs after receiving SKF96365 and mechanical stretching assessed by Annexin V/PI double staining; n=3. Data represented as mean ± S.D. (t test, two-sided). *, P<0.05.