(A) Blood samples were taken from ten PMR patients longitudinally through their steroid treatment course over 6 months: samples were taken prior to steroid commencement (pre) and again at 1-, 4-, 12- and 24-week post-steroid. Whole blood flow cytometry was carried to determine neutrophil phenotype with respect to annexin-A1 (AnxA1) (A), ALX/FPR2 (B), CD62L (C) and CD11b (D). Right hand histograms are from representative samples. Dotted lines refer to age-mated controls. Data are mean ± S.E.M. of n=10 per time-point. ^*P<0.05; ^**P<0.01 compared with age-matched control (n=9); ^§§P<0.001 compared with week 12. One-way ANOVA, Bonferroni post-correction, repeated measures.