H&E staining and analysis of the area of the new bone formation
(A) Group A presented extensive osteonecrosis, diffuse distribution of the empty bone lacuna, and fibrous tissue hyperplasia around the necrotic bone; (B) Group B had a few new bone, and the transplanted bone was not absorbed completely; (C-D) In groups C and D, a large number of new bone formation was observed, and the graft bone was absorbed; (E) In group E, a large number of new bone trabeculae were found, which were not clear with normal bone boundaries, and the repair was close to the normal cancellous bone; (F) At 12 weeks after the operation, the quantitative analysis and comparison of new bone revealed that group E had the largest and significant difference. Compared with groups A, B, C and D, #P<0.05 (P<0.05).