Alteration of miR-543 expression changed PTEN protein expression
(A) Protein expression levels of PTEN were assayed by Western blotting in HCT8 and HCT8/FU cells. (B) Protein expression levels of PTEN in miR543-inhibitor-transfected HCT8/FU cells and miR543-mimic-transfected HCT8 cells. (C) The qRT-PCR analysis of miR-543 expression in HCT8 and HCT8/FU cell lines transfected with PTEN and pCMV6 (NC).