(A) Relative level of miR-543 in HCT8/FU cells transfected with the miR-543 inhibitor or inNC. (B) Dose–response curves of HCT8/FU cells transfected with the miR-543 inhibitor and its control towards 5-FU. IC₅₀ values were listed in the tables below. (C) Dose–response curves of HCT8 cells transfected with the miR-543 mimic and its control towards 5-FU. IC₅₀ values are listed in the tables below. (D). Protein expression levels of Bcl-2 and BAX in miR543-inhibitor-transfected HCT8/FU cells and in miR543-mimic-transfected HCT8 cells. (E) Protein expression levels of Bcl-2 and BAX in HCT8 cells and HCT8/FU cells. (F) HCT8/FU cells transfected with the miR-543 inhibitor and inNC and HCT8 cells transfected with the miR-543 mimic and inNC were treated with 5-FU for 24 h, followed by analysis of apoptosis. ^*P<0.05 vs control; ^**P<0.01 vs control; ^***P<0.001 vs control. The data are presented as the mean ± S.D. of triplicate experiments.