(A) Influence of Akirin1 ectopic expression on MEF2A mRNA expression. (B) Influence of Akirin1 ectopic expression on MEF2B mRNA expression. (C) Influence of Akirin1 ectopic expression on MEF2C mRNA expression. (D) Influence of Akirin1 ectopic expression on MEF2D mRNA expression. After transfecting with duck pEGFP-N1-Akirin1 or pEGFP-N1 plasmids for 24 h, myoblast differentiation was induced by switching the cells to DM for 12–48 h. Each point represents the relative mean ± S.D. *, P<0.05.