At 24 h post-transfection in the GM, RT-PCR and Western blot were carried out. (A–D) Influence of Akirin1 ectopic expression on p38α, p38β, and p38γ mRNA expression and p38α protein expression. (E–G) Effects of SB203580 and Akirin1 ectopic expression on the expression of p38α and MRF4. The values below each Western blot image represent the relative abundance of target protein compared with GAPDH. Each point represents the relative mean ± S.D. *, P<0.05.