Figure 4
(A) qRT-PCR analysis was used to verify the stability of miR-145 knockdown or overexpression. (B) The results of Matrigel assay were plotted as relative tube length. (C) The results of migrated BMECs under OGD stimulation were expressed as the number of migrated cells per field (magnification: ×200; scale bar: 100 μm). (D) Matrigel assay was used to evaluate the effects of miR-145 on angiogenesis in BMECs. (E) Migration of BMECs was measured by Transwell migration assay. BMECs were treated under OGD conditions for 12 h, grouping as OGD(−), OGD(12 h), mimic NC, miR-145 mimic, inhibitor NC, and miR-145 inhibitor. Data represent means ± S.D. based on three independent experiments; *P<0.05, **P<0.01.
miR-145 inhibited angiogenesis and cell migration in BMECs under OGD conditions

(A) qRT-PCR analysis was used to verify the stability of miR-145 knockdown or overexpression. (B) The results of Matrigel assay were plotted as relative tube length. (C) The results of migrated BMECs under OGD stimulation were expressed as the number of migrated cells per field (magnification: ×200; scale bar: 100 μm). (D) Matrigel assay was used to evaluate the effects of miR-145 on angiogenesis in BMECs. (E) Migration of BMECs was measured by Transwell migration assay. BMECs were treated under OGD conditions for 12 h, grouping as OGD(−), OGD(12 h), mimic NC, miR-145 mimic, inhibitor NC, and miR-145 inhibitor. Data represent means ± S.D. based on three independent experiments; *P<0.05, **P<0.01.

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