LncRNA-MALAT1 up-regulated VEGF-A and ANGPT2 via targetting miR-145
(A) qRT-PCR analysis was performed to measure relative expression level of VEGF-A in BMECs. (B) qRT-PCR analysis was performed to measure relative expression level of ANGPT2 in BMECs. (C) qRT-PCR analysis was used to measure the effects of MALAT1 on the expression level of miR-145 in BMECs. (D) Western blot analysis was performed to measure protein levels of VEGF-A and ANGPT2, GAPDH was used as an internal control. (E) Conservation of MALAT1 at the binding site of miR-145 was snapshot, 5 nts on 3′-UTR of MALAT1 were replaced in constructed MALAT1 mutant. (F) Dual-luciferase reporter assay was used to measure relative luciferase activity. BMECs were treated under OGD conditions for 12 h, grouping as OGD(−), OGD(12 h), si-control, si-MALAT1, pcDNA-control, and pcDNA-MALAT1. Data represent means ± S.D. based on three independent experiments; *P<0.05, **P<0.01.