(A) The overview of genome editing system for KI. The sequencing confirmation of T124A (B); T1976C (C); C2243T (D); KI hESCs and KO (E); and WT (F) hESCs. All KI hESCs are heterozygous and KO is homozygous. (G) Two colonies each of WT and KO hESCs were subjected to Western blotting for ADAM17. GAPDH was used as loading control. (H) Immunofluorescent staining of WT, KO, Y42D (T124A), L659P (T1976C), and A748V (C2243T) hESCs with pluripotency marker octamer-binding transcription factor 4 (OCT4) (green) and Tra1-60 (red). DAPI was used for nucleus staining (blue) (N=4).