Repsox, an inhibitor of TGF-β type I receptor, reduces the expression of Cx43
(A) qPCR showing down-regulated mRNA expression of Cx43 after being treated with Repsox. The results were based on the three independent experiments (n=3). **P<0.01. (B) Western blot assay showing the expressions of Cx43 upon exposure to Repsox for 24 h. Quantitation was performed to confirm the protein changes (n=3). **P<0.01. (C) Representative IF staining by CLSM showing the decreased Cx43 in osteocytes in response to Repsox (cytoskeleton, green; Cx43, red; nucleus, blue). The results were based on three independent experiments (n=3). (D) Quantitation of GJ number by ImageJ was performed to confirm the changes after Repsox treatment (n=3). *P<0.05.