Effect of pretreatment with FM0807 on TNF-α, IL-6, IL-1β, and iNOS gene expression in LPS-induced RAW 264.7 cells
(A) The expression of TNF-α mRNA. (B) The expression of IL-1β mRNA. (C) The expression of IL-6 mRNA. (D) The expression of iNOS mRNA. RAW264.7 cells were pretreated with FM0807 (10, 20, 40 μM) and hydrocortisone, respectively, for 20 h prior to LPS stimulation. After 4 h of LPS stimulation, the TNF-α, IL-6, IL-1β, and iNOS mRNA levels were accessed by quantitative-RT-PCR. Data are presented as mean ± S.D. Representation of results obtained from three independent experiments are shown. **P<0.01 compared with the control group; #P<0.01 and ##P<0.05 compared with the LPS-treated group. Abbreviation: RT-PCR, real-time PCR.