Figure 4
SIRT1 reduced proinflammatory cytokine production and NF-κB-related protein expression in RA-FLS
RA-FLS was transfected with pCDNA3.1 or pCDNA3.1-SIRT1. TNF-α (A), IL-6 (B), IL-8 (C), and IL-1β (D) concentration in the supernatant of transfected cells were detected by ELISAs. Protein expression of NF-κB p65, p-p65 (Ser536), and Ac-p65 (Lys310) were detected by Western blot (E) and quantified using GAPDH as loading control (F); N = 6, mean ± SD, **P < 0.01.
