Phosphorylation of AMPKThr172 was assessed by Western blot of myotubes after treatment
(A) 2 mM metformin for 16 h (B) 1 µM 991 for 2 h. Densitometry is expressed as a ratio of phosphorylated to total protein. Open bar: untreated, closed bar: treated. *P<0.05, **P<0.01. n=7 controls and 8 CFS.