TEBC is associated with changes in Syn immunoreactivity in the cerebellar IN
(A) Location of guinea pig cerebellar IN. Left: a representative sample of the coronal cutting surface of the guinea pig cerebellum; the black arrow indicates the location of the cerebellar IN. Right: a schematic drawing of the coronal section; the location of the cerebellar IN is indicated by the black arrowhead. (B) CS, US, and analysis periods during TEBC. In a CS-US paired trial, we analyzed the parameters of SR (1–120 ms period after the CS onset), eyeblink CR (121–450 ms period after the CS onset), and UR (1–500 ms period after the US onset). These responses were based on the average amplitude of baseline (1–400 ms period prior to the onset of the CS). An example of a typical SR, CR, and UR is illustrated by the red curve. (C) Percentage of CRs in sessions 2, 6, and 10. The trace-conditioned group (n=4) showed a large increase in CR percentage. (D) Syn immunoreactivity changes in the cerebellar IN following the second (a,b), sixth (c,d), and tenth (e,f) sessions. (a), (c), and (e) represent the pseudoconditioned trace-conditioned group (n=4); (b), (d), and (f) represent the trace-conditioned group (n=4). (E) Syn immunoreactivity staining in the cerebellar IN following the second, sixth, and tenth session. The trace-conditioned group shows an increase in Syn expression with time; and at sessions 6 and 10, the Syn expression was significantly higher in the trace-conditioned group than in the pseudoconditioned group. *P<0.05; **P<0.01.