mTOR inhibition is involved in DEX-suppressed protein synthesis
The effect of DEX (100 μmol/l for 24 h) on protein synthesis (A), and the protein expression of mTOR, P-mTOR (B), p70s6k, P-p70s6k (C), 4EBP1, P-4EBP1 (D) in C2C12 myoblasts. The protein level was quantified by normalizing total proteins with β-actin, and by normalizing phosphorylated proteins with their total pairs. The values shown are the means±S.E.M. (n=6); a, b: means with different letters are significantly different (P<0.05).