The myoblasts were incubated with or without DEX (100 μmol/l) for 24 h. At 23 h of the DEX exposure, leucine (5, 10 or 15 mmol/l) was added to DEX treated cells for the following 1 h. After this, all cells were immediately subjected to an additional 30-min puromycin exposure (1 μmol/l) and then the detection of protein synthesis (A), or were directly collected for mRNA and protein analysis (B).