pDCs and mDCs from HD and RR- MS patients were activated for 24 h with resiquimod and co-cultured with autologous naive CD4+ T-cells (ratio 1:1) in the presence of anti-CD3-CD28-coated beads for 6 days, then re-stimulated for 24 h. Protein expression of T-cell-derived cytokines IFN-γ (A), IL-13 (B), IL-17 (C), IL-10 (D), IL-22 (E) and IL-9 (F) was analysed by Flow Cytomix or by ELISA. A paired Student's t test was used to compare no cytokines and Th9 cocktail. An unpaired Student's t test was used to compare HD and RR-MS. *P<0.05, **P<0.005.