Atf3 is required for cardiomyocyte hypertrophy induced by ET-1
Cardiomyocytes were uninfected (A and B) or were infected with No-AS (C and D) or AS-Atf3 (E–H) AdVs, then unstimulated (Control; A, C, E and G) or exposed to 100 nM ET-1 for 24 h (B, D, F and H). Cells were immunostained with antibodies to troponin T. Results are representative of three independent experiments. Scale bar=50 μm.