Cardiomyocytes were uninfected or were infected with No-AS (○ and broken line) or AS-Atf3 (● and solid line) AdVs, then exposed to 100 nM ET-1 for the times indicated. (A) Nuclear extracts (20 μg of protein) were immunoblotted for Egr1 (upper images) or Atf3 (lower images). Representative blots of the same nuclear extracts are shown. Positions of relative molecular mass markers are given in kDa on the left-hand side. (B) Densitometric data for expression of Atf3 protein. (C) Densitometric data for expression of Egr1 protein. Results were normalized to the no-virus samples on each blot and are means±S.E.M. for three myocyte preparations. *P<0.05 for AS-Atf3 relative to No-AS at the same time point (one-way ANOVA with SNK post-test).