Results were validated for Egr1 (A), Ptgs2 (B), Areg (C), Dusp5 (D), Dusp1 (E), Il1rl1 (F) and IL6 (G). Left-hand panels: cardiomyocytes were infected with No-AS (○ and broken line) or AS-Atf3 (● and solid line) AdVs, then exposed to 100 nM ET-1 for the times indicated. RNA was extracted and expression of mRNAs analysed by qPCR. Results are means±S.E.M. for three myocyte preparations. *P<0.05 for AS-Atf3 relative to No-AS at the same time point (one-way ANOVA with SNK post-test). Right-hand panels: microarray data for each of the mRNAs studied are presented relative to uninfected (NV) controls. Results are shown for unstimulated cells (open bars) and cardiomyocytes exposed to ET-1 for 90 min (filled bars). Results are means for three independent myocyte preparations and hybridizations.