Neutrophils were isolated from 12 healthy donors and treated with or without 100 nM MLi-2 for 30 min. Cells were then lysed and 10 µg of whole cell extract subjected to electrophoresis in which the Phos-tag acrylamide was polymerised into the polyacrylamide gel in order to retard the electrophoretic mobility of LRRK2-phosphorylated Rab10. The gels were subjected to immunoblot analysis with an antibody that specifically recognises Rab10 (1 µg/ml antibody), and the band corresponding to phosphorylated and non-phosphorylated Rab10 is marked with open and filled circles, respectively. A low (top panel), medium (middle panel) and high exposure (bottom panel) of the immunoblot are shown. Similar results were obtained in two separate experiments.